Decellularized matrices are created by:

Decellularized matrices are produced through a process that involves exposing tissues to detergents. This method effectively removes cellular components while preserving the extracellular matrix (ECM), which is critical for applications in tissue engineering and regenerative medicine. The detergents break down cell membranes and facilitate the elimination of cellular contents, including nucleic acids and proteins, resulting in a matrix that retains the structural and biochemical properties of the original tissue.

This preserved ECM can then serve as a scaffold for new cells in regenerative applications, enabling better integration and functionality when the matrix is used in the body. In contrast, stimulating cell growth, artificially creating tissues, or incorporating synthetic materials do not yield decellularized matrices since they involve either maintaining living cells, forming tissues de novo, or using non-biological components, rather than focusing on the removal of cells from existing tissue.